FastQCFastQC Report
Mon 27 Feb 2023
SRR3128480.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128480.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280872
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAACGGAA28541.0161212224785667No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26610.9474066478680679No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG23550.8384602238742203No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC18370.6540345780284258No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA13540.48207012446950925No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG12360.4400581047594634No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATG9210.32790737417756133No Hit
CGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT8380.2983565467543935No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG7060.2513600501295964No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG6580.23427041499330656No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA6330.22536956335982225No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG6150.21896095018371356No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGAACGGAATC5960.2121963029422655No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCGAACGGAATCG5930.2111282007462474No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGG4280.1523825799652511No Hit
ACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG4150.14775413711583923No Hit
AGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT3930.1399213876783731No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC3920.13956535361303368No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT3570.12710416132615568No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT3410.12140761628072574No Hit
GACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC3400.12105158221538638No Hit
GATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC3360.11962744595402888No Hit
GGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT3230.11499900310461704No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCGAACGGAATCGT3210.1142869349739383No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGC3180.11321883277792019No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGG3160.11250676464724145No Hit
ATCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC3070.10930245805918709No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGG2980.10609815147113275No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCC2930.1043179811444359No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT2860.1018257426870603No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGG301.3144017E-894.081431
ATGGGTA700.093.997694
GCGGTAC156.8974117E-493.997695
TAAGGGT156.8974117E-493.997693
CGTTTTT20250.088.041631
GATTAGG600.086.241311
CGAGGGA1050.085.0455253
GTAAGGG3000.083.031292
AGGGTGC401.031367E-982.247985
AGGGTAC1200.082.247985
AGTAAGG1550.081.9418951
GGGTACC750.081.464666
ATAGAGG1450.081.104681
ATAGCGG353.8307917E-880.641231
TACGCGG353.8307917E-880.641231
AGGGAAT7700.080.569445
TAAGGGA2000.079.8980263
ACTAGGG1300.079.53652
ATAGGGC1550.078.836783
GGTAAGG1200.078.401191
Produced by FastQC (version 0.11.5)