FastQCFastQC Report
Mon 27 Feb 2023
SRR3128479.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128479.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047529
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173200.8458976649414979No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG27340.13352680230658517No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG27140.13255001516462037No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC23320.11389338075309312TruSeq Adapter, Index 22 (95% over 22bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAAAGGTCC21370.10436970611893653No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA20780.10148818405014044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57800.079.860721
CGTAGGG5500.078.646152
AGTAAGG13300.071.818511
GGTAAGG12200.071.351631
AGTAGGG30700.071.0611042
TAGGGCA9150.070.365684
ATAGGGC10500.070.2702943
ACGGGTA2750.070.066854
GTAGGGA15200.068.638983
TATAGGG15650.067.29552
AGATAGG13100.066.80881
ATAGGGA17050.066.704083
ATAGCGG6250.066.251341
CCGTACA13350.066.181713
GTATAGG8250.066.160081
TCCGTAC13800.066.095972
AGGGATG25550.065.6657265
TAAGGGA16750.065.65423
ATAAGGG25250.065.5441062
ACTAGGG7400.065.442192
Produced by FastQC (version 0.11.5)