FastQCFastQC Report
Mon 27 Feb 2023
SRR3128478.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128478.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1980238
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158640.8011158254714836No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG32790.16558615681549388No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG32030.1617482343031494No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAAAGGTCC29170.14730552590143203No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC28180.1423061268392991TruSeq Adapter, Index 22 (95% over 22bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA24180.12210653466906503No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54150.079.622531
CGTAGGG4850.076.551382
ACGGGTA3050.073.962074
GAATAGG11350.072.494871
TAGGGCA8000.072.257484
GGTAAGG11050.071.9100341
AGTAAGG12200.071.297941
CCGTACA13600.070.4950943
TAAGGGA16600.070.211983
ATAGGGC9700.069.768343
ACTAGGG8650.069.544292
AGTAGGG29350.069.1740652
AGGGTAC9050.069.063535
TCCGTAC14750.068.185082
TATAGGG14000.067.809562
ATCCGTA15100.067.569061
CGTACAC14200.067.185474
GATAGGG29400.066.818482
AGGGATG21600.066.575345
CATAGGG10050.066.403342
Produced by FastQC (version 0.11.5)