FastQCFastQC Report
Mon 27 Feb 2023
SRR3128477.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128477.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2192750
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198860.906897731159503No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG36670.16723292669022916No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG35730.16294607228366206No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC30860.14073651807091553TruSeq Adapter, Index 22 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21960.10014821571086535No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG5650.079.874022
CGTTTTT64150.079.5778961
GAATAGG12850.073.595671
TAGGGCA11100.073.24824
TAGGGTA7200.069.843354
AGTAGGG31850.069.222322
GGTAAGG12650.069.180191
TAGAGGG40350.068.853612
ATAGGGC11000.068.78713
GTAGGGT7700.067.74953
GCATAGG5750.067.097351
AGGGTAC10600.066.949135
GGATAGG11650.066.63731
AGGGATG27200.066.522065
ATAGGGA19350.066.306413
ATAAGGG28900.066.2032552
ATAGAGG15900.065.988331
TACGACC5000.065.796366
AGTAAGG12850.065.540431
AAGGGGC66750.065.127494
Produced by FastQC (version 0.11.5)