FastQCFastQC Report
Mon 27 Feb 2023
SRR3128476.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128476.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2250573
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191540.8510721491815639No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC49970.22203234465178423No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG29170.12961143673189005Illumina PCR Primer Index 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG28930.12854504164050667Illumina PCR Primer Index 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC28060.12467935943424185Illumina PCR Primer Index 2 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64050.082.446031
AGGGATG43850.077.5939255
CGTAGGG6350.077.0190662
GACCGAT2150.076.504748
GAATAGG14250.076.294481
ATAGGGC14200.074.796083
TAGGGAT19350.073.590274
GTAGGGA17400.072.654383
ACGGGTA1500.072.060184
GAGGGAT34500.071.7877354
ATAACGG2000.070.597171
AGTAGGG33650.069.875282
CCGTACA13600.069.802543
TAGAGGG41600.069.5217062
TAGGGCA13050.069.503324
ATAGGGA24250.069.185523
AGATAGG12450.068.801521
AGGGAAT29200.068.562325
TAAGGGA24300.068.269583
AGGGCAT21150.068.216085
Produced by FastQC (version 0.11.5)