Basic Statistics
Measure | Value |
---|---|
Filename | SRR3128475.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2112494 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 17458 | 0.826416548402031 | No Hit |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 4626 | 0.2189828704838925 | No Hit |
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG | 3558 | 0.16842651387412225 | Illumina PCR Primer Index 2 (95% over 23bp) |
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG | 3278 | 0.15517203835845214 | Illumina PCR Primer Index 2 (95% over 23bp) |
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC | 3133 | 0.14830811353783727 | Illumina PCR Primer Index 2 (95% over 24bp) |
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCCTGTAAC | 2767 | 0.13098262054235418 | No Hit |
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA | 2354 | 0.1114322691567408 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 5665 | 0.0 | 81.77095 | 1 |
CGTAGGG | 730 | 0.0 | 75.3212 | 2 |
AGGGATG | 4025 | 0.0 | 74.26365 | 5 |
CCGTACA | 1545 | 0.0 | 74.21908 | 3 |
ATAGGGC | 1095 | 0.0 | 73.81906 | 3 |
TAGGGCA | 1035 | 0.0 | 73.55957 | 4 |
GAATAGG | 1255 | 0.0 | 73.07244 | 1 |
TCCGTAC | 1620 | 0.0 | 72.81367 | 2 |
CGTACAC | 1585 | 0.0 | 72.347755 | 4 |
ACGGGTA | 275 | 0.0 | 71.776306 | 4 |
GGTAAGG | 1450 | 0.0 | 71.678185 | 1 |
AGTAGGG | 3220 | 0.0 | 71.66051 | 2 |
ATAGAGG | 1300 | 0.0 | 71.62829 | 1 |
GTAGGGC | 1125 | 0.0 | 71.432816 | 3 |
ATAGGGA | 2055 | 0.0 | 70.207054 | 3 |
AGATAGG | 1075 | 0.0 | 69.558685 | 1 |
TAAGGGA | 2075 | 0.0 | 69.530365 | 3 |
TAGGGAT | 1740 | 0.0 | 69.41421 | 4 |
GTAGGGA | 1985 | 0.0 | 69.36834 | 3 |
GAGGGAT | 2970 | 0.0 | 69.149574 | 4 |