FastQCFastQC Report
Mon 27 Feb 2023
SRR3128475.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128475.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112494
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174580.826416548402031No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC46260.2189828704838925No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG35580.16842651387412225Illumina PCR Primer Index 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG32780.15517203835845214Illumina PCR Primer Index 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC31330.14830811353783727Illumina PCR Primer Index 2 (95% over 24bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCCTGTAAC27670.13098262054235418No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA23540.1114322691567408No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT56650.081.770951
CGTAGGG7300.075.32122
AGGGATG40250.074.263655
CCGTACA15450.074.219083
ATAGGGC10950.073.819063
TAGGGCA10350.073.559574
GAATAGG12550.073.072441
TCCGTAC16200.072.813672
CGTACAC15850.072.3477554
ACGGGTA2750.071.7763064
GGTAAGG14500.071.6781851
AGTAGGG32200.071.660512
ATAGAGG13000.071.628291
GTAGGGC11250.071.4328163
ATAGGGA20550.070.2070543
AGATAGG10750.069.5586851
TAAGGGA20750.069.5303653
TAGGGAT17400.069.414214
GTAGGGA19850.069.368343
GAGGGAT29700.069.1495744