FastQCFastQC Report
Mon 27 Feb 2023
SRR3128474.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128474.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2358526
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219550.9308780144887103No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC53930.22865976461569643No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG38230.1620927647183029Illumina PCR Primer Index 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG38150.16175356981436712Illumina PCR Primer Index 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC35110.1488641634648081Illumina PCR Primer Index 2 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71900.083.246471
TAGGGCA13100.076.415524
CGTAGGG6750.075.20692
AGTAGGG37750.074.957882
AGGGATG45300.074.4902655
ATAGGGC15350.074.093523
GTAGGGC12550.072.649283
GAATAGG13500.072.15131
GTAGGGA20600.070.952293
TAGGGAT20750.070.665884
GAGGGAT34050.070.530514
TAGAGGG42400.069.952182
ATGCGGG9550.068.906852
GAGGGTA12500.068.80414
ATAGGGA25500.068.376523
ACGGGTA2350.067.996154
AGGGAAT29250.067.3226855
AAGGGAT48250.066.916424
AGGGATA25400.066.240345
AGGGCAT20100.066.170395
Produced by FastQC (version 0.11.5)