FastQCFastQC Report
Mon 27 Feb 2023
SRR3128473.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128473.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2217497
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT469112.115493279134087No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTT42250.1905301337498991No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCT39710.17907577778008268No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT36810.16599796978304818No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG32650.14723807969075042No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC25570.11531018982212829TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT152300.087.018311
AGGGATG35050.071.7342155
GTAGGGC9300.070.746613
ATAGGGC13050.070.2238543
CGTAGGG6800.069.831172
AGTAGGG34050.069.452482
GAATAGG13050.068.520581
GAGGGAT26900.068.310124
TAGGGCA11050.068.0484544
AGAGGGC34550.067.739483
TAGGGCG4250.067.4530264
TAGAGGG39100.067.336132
ATAGGGA20450.067.334073
AAGAGGG75050.066.0277942
CCGTACA7150.065.728623
TCCGTAC7300.065.692252
AAGGGAT34900.065.5788964
GTACGGG5600.065.4852452
CGAAGGG22750.065.304422
TAGGGTA6500.065.0713354
Produced by FastQC (version 0.11.5)