FastQCFastQC Report
Mon 27 Feb 2023
SRR3128472.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128472.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133019
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT438502.055771655104807No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTT44500.20862448951462692No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCT42130.19751347737643216No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT40320.18902785207257883No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG37970.17801060374989627No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC29330.13750463544862937TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT139550.087.1168061
ATAGCGG4550.073.386841
AGGGATG29900.072.457825
AGTAAGG12600.069.424641
GAATAGG10600.069.213371
CGTAGGG5850.069.0856552
ATAGGGC11850.069.004323
AGTAGGG31900.068.208052
GGTAAGG14150.067.8023761
CCGTACA7150.067.6981053
TAGGGCA9950.067.5395664
TCCGTAC7400.066.681122
AGGGAAT21650.065.9889455
TAGGGAT12800.065.718614
ATAGAGG14850.065.5564041
ACGGGAT7100.065.527294
ATAGGGA18750.065.416093
ATGAGGG34600.065.194432
AGAGGGC30650.065.163413
TAGAGGG37150.064.89392
Produced by FastQC (version 0.11.5)