FastQCFastQC Report
Mon 27 Feb 2023
SRR3128471.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128471.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2379645
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT534842.2475621363690803No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTT48040.20187885167745606No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCT46450.1951971827730607No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT44160.1855738986277365No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG42460.17842997589976656No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC32010.13451586266018672TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT161350.087.5857851
ATAGCGG5650.073.296211
ATAGGGC13900.071.6806953
AGGGATG34600.071.176455
AGTAGGG35400.071.171572
GTAGGGC11000.069.215523
TAGGGCA10450.068.810754
GGTAAGG13600.068.513091
GAGGGAT26000.067.243574
CGTAGGG8000.066.982282
TATAGGG19400.066.388662
GAATAGG13700.066.29551
TAAGGGA19550.065.869583
AGGGAAT24850.065.2495
TAGAGGG40600.065.181952
TAGGGTA6950.064.9183654
AGTACGG2900.064.909651
AAGGGAC23050.064.227474
AGTAAGG14700.063.7063981
AATAGGG31750.063.6604652
Produced by FastQC (version 0.11.5)