FastQCFastQC Report
Mon 27 Feb 2023
SRR3128470.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128470.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2696003
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267230.9912080958366886No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28700.10645388747712817No Hit
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC27190.10085300350185071TruSeq Adapter, Index 8 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90050.082.5186541
CTACGAC6500.073.7496955
ATAGCGG6100.070.9760361
CGTAGGG8300.070.246792
AGTAGGG43800.070.207992
TAGGGCA14400.070.169664
ATAGGGA24550.069.682443
ATAACGG2400.068.629451
ATAGGGC15950.068.3597953
AGGGATG40550.068.033175
GAATAGG16350.067.9278641
CGAAGGG27800.066.978172
TAGCGGG14850.066.4929662
TAGAGGG44350.066.3687442
TATAGGG20100.065.5005342
GTAGGGC11500.065.3876343
TAGACGG3550.064.956331
AGTAAGG19650.064.6628651
ACGGGAT8450.064.517084
AGAGGGC36250.064.4353
Produced by FastQC (version 0.11.5)