FastQCFastQC Report
Mon 27 Feb 2023
SRR3128469.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128469.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2542026
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250020.9835461950428516No Hit
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC30190.11876353743038033TruSeq Adapter, Index 8 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG29150.11467231255699194TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG29150.11467231255699194TruSeq Adapter, Index 13 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27490.10814208823985277No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT82850.082.542291
TAGGGCG2900.081.029534
AGGGATG40000.073.669455
ATAGGGC13950.071.7576453
TAGGGCA12950.070.042054
AGTAGGG43950.069.612132
GAATAGG15100.068.836681
GTAGGGC12300.068.392853
CGTAGGG8050.068.305042
CGAAGGG23950.067.894312
TAGAGGG39950.067.6415942
TAGGGAT19200.067.31364
ATAGGGA22950.067.166693
CTACGAC8250.066.651795
TAGCGGG14500.066.11882
AAGGGTA17300.065.741654
TAGGGTA8700.065.363824
ATAGAGG17000.065.293161
AGGGAAT29050.064.7133265
AGGGTAC10850.064.54115
Produced by FastQC (version 0.11.5)