FastQCFastQC Report
Mon 27 Feb 2023
SRR3128468.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128468.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2856031
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303011.0609478678627788No Hit
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC33610.11768079548156166TruSeq Adapter, Index 8 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG32970.1154399234462091TruSeq Adapter, Index 13 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31710.11102820662660874No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG31480.11022289323890393TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96200.083.8211061
ATAGGGC16950.075.9739763
ATAGGGA26900.071.633413
TAGGGCA14550.071.386044
TAGAGGG44900.070.248372
AGGGATG40400.070.032425
GTAGGGC12900.069.951263
GAATAGG17450.069.287561
GTACGGG5700.069.273082
TAGGGCG4850.067.832894
TAGCGGG15600.067.798172
CGTAGGG9300.067.7301252
AGTAGGG47150.067.593992
CGAAGGG28900.067.175712
AGGGTAC13250.067.0393755
ATAGAGG20150.066.307231
AGGGAAT30900.066.010845
ATAAGGG40850.065.820932
ACGGGAT8750.065.529344
TAAGGGA26900.064.994213
Produced by FastQC (version 0.11.5)