FastQCFastQC Report
Mon 27 Feb 2023
SRR3128467.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128467.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748429
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160160.9160223263283782No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTTGAAGG47980.274417777330392No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG44340.2535990880956562No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC40730.23295198146450327Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG37480.21436386607634625No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA21550.12325350357377968No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT56250.082.465181
CGTAGGG3900.079.5718462
ATAGCGG2550.075.6414641
ACGGGAT5200.074.1125644
GAATAGG11050.071.525891
TAGGGCA7900.070.199864
TAGCGGG7500.069.5891952
ATAGAGG10850.069.375561
AGTAGGG23800.068.94912
TAGGGCG3300.068.361044
TAGGGTA7050.067.997414
TACACGG1250.067.745231
AAGGGAT27350.067.533084
AGGGCGA10650.067.5185555
ATAGGGA17700.067.443763
GGGCGAT8450.067.299226
AGGGCAC11950.067.252675
AGGGTAC10000.067.207445
AGGGATG21050.067.20415
TAAGGGA14900.066.869943
Produced by FastQC (version 0.11.5)