FastQCFastQC Report
Mon 27 Feb 2023
SRR3128466.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128466.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1667438
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147700.8857900563619157No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTTGAAGG62050.37212777926375673No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG49210.2951234168826667No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC44640.26771610098846255Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG42210.2531428454911067No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA25570.15334903006888412No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT53300.081.9410251
TAGGGCG2950.076.4650044
ATAGGGC8400.074.966813
TAGGGCA6950.073.703014
ACGGGAT5200.073.2023854
AGGGCAT15350.073.1721955
AGTAGGG22400.073.008722
GAATAGG10600.071.849211
ATAGCGG2950.071.7139051
CCGTACA25700.071.131183
CGTACAC25700.070.7654654
TAGGGAT10900.070.706754
TCCGTAC27150.070.2748262
GGGCGAT8150.069.772516
AAGGGCA22500.069.760164
AGGGTAC8300.069.643985
ATCCGTA27550.069.6227951
AGGGATG17300.068.999225
GGTAAGG7850.068.871741
ACGGGTA1650.068.355094
Produced by FastQC (version 0.11.5)