FastQCFastQC Report
Mon 27 Feb 2023
SRR3128465.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128465.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873504
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186640.9962081746289306No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG55800.2978376347208226No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC50060.2671998565255265Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG48510.2589265888943925No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTTGAAGG33300.17774181426887797No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64650.083.896461
AGGGTAC10050.077.162545
ATAGCGG3600.073.175951
TAGGGCA8050.072.395974
TAGCGGG8450.071.199452
AGTAGGG27650.071.056622
CGTAGGG4800.070.504142
GAATAGG11550.070.053411
AAGGGTC11700.068.289154
ATAGGGC9300.068.224353
AGGGCAC12750.068.1946265
GGGCGAT8550.068.162296
CGAAGGG14850.068.051142
GTAGGGC7950.067.985983
AGGGCAT18050.067.959775
AGGGATG21500.067.547395
GGTAAGG8850.067.506141
AAGGGAC16600.067.100984
CGAGGGA9450.066.644083
TAGGGTA8350.065.854894
Produced by FastQC (version 0.11.5)