FastQCFastQC Report
Mon 27 Feb 2023
SRR3128464.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128464.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2721876
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236790.8699514599489471No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG41860.15379098827426377No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC36970.13582543804346708No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG33530.12318709595881665No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33500.12307687785924121No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83900.080.942121
CGTAGGG7950.079.8519442
TAGGGCA12750.074.826834
ATAGCGG4600.074.6853261
AGTAGGG41400.071.558142
ATAGGGA23100.071.2077943
GTAGGGA21650.070.984143
GTAGGGC12100.070.689923
TAGGGCG5000.070.495714
AGTAAGG16700.069.888451
ATAGGGC15700.069.747353
TATAGGG19700.069.700432
GAATAGG17100.069.35451
GTAGGGT10200.069.113443
TAGGGAT17600.067.5583954
AGGGATG39300.067.446285
TAAGGGA23650.067.167163
AATAGGG37400.067.015382
TACGACC8700.066.444246
ACGGGTA3200.066.089744
Produced by FastQC (version 0.11.5)