FastQCFastQC Report
Mon 27 Feb 2023
SRR3128463.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128463.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624002
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215430.8209978498491998No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG47780.18208827584735074No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC42600.16234743723518502No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG39740.15144805529873834No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGTCAAAG33530.12778191480036982No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32000.12195112656164134No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA26710.10179108095192001No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68750.081.799731
ATAGGGC15900.078.329773
CGTAGGG6500.072.304412
TATAGGG19700.072.2860642
ACGGGTA2350.071.996734
TAGGGCA12150.071.947354
ATAGGGA22250.070.972053
CCGTACA16500.070.924053
GTATAGG11700.070.732691
TAGGGCG5600.068.8183064
AATAGGG33400.068.808252
AGTAGGG40550.068.72932
AGGGATG35700.068.457585
GATAGGG35300.068.433152
CGTACAC17150.067.687894
ACGGGAT8750.067.139814
AAGGGAT43100.067.061924
GGTAAGG12300.066.900031
GTAGGGA19200.066.580313
ATAGCGG4100.066.517741
Produced by FastQC (version 0.11.5)