FastQCFastQC Report
Mon 27 Feb 2023
SRR3128462.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128462.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2909298
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271140.9319774048584917No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG51820.178118570184285No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC48380.16629441191655167No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG46180.158732450233699No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36890.12680034840019824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85950.081.921321
ATAGCGG7200.074.472061
ACGGGTA4000.074.0228044
ATAGGGA23000.071.723963
TAGGGCA13950.071.424414
GTAGGGA22950.070.8562853
GTAGGGC13950.070.413683
AGTAGGG45850.069.719072
TAGGGCG4900.069.059184
GACCGAT2600.068.690278
ATAGGGC17600.068.628653
TAAGGGA25350.068.226783
TAGGGAC12650.067.990084
CGTAGGG7600.067.420962
TAGGGTC8000.066.9730154
TAGACGG4150.066.869011
AAGAGGG91550.066.855122
TATAGGG20050.066.820922
TGTAGGG20900.066.802422
TAGAGGG52100.066.0473562
Produced by FastQC (version 0.11.5)