FastQCFastQC Report
Mon 27 Feb 2023
SRR3128461.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128461.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782752
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69660.8899370426393034No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATGCTGTG50330.6429878173418913No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG33000.42158946895057436No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30690.3920782061240342No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC29110.3718930133682188TruSeq Adapter, Index 15 (95% over 21bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA24670.31517006663668695No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG23330.29805097910960304No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATG17600.22484771677363968No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCATGCTGTGTC11730.14985589305424962No Hit
CGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCT11320.14461796328850007No Hit
ACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG8940.11421241977024651No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA8930.11408466538571603No Hit
AGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCT8060.10297003393156454No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT36750.086.5325241
AGGGATG15700.083.517645
TAACGGG2500.082.731252
AGGGAAT14750.081.568345
TAAGGGA7400.080.022573
GGAGCTA24300.079.8762059
CGTAGGG1950.079.549282
ATAGCGG1200.078.404081
CGCTACG5700.078.328973
TAGCGGG3550.078.123292
AGGGCTA3950.077.337465
AGGGCAT6200.077.3182755
GGAATGC10950.076.397577
GGGAATG19550.076.2054756
GGATGGC13450.075.8247767
GAGGGTA4350.075.627974
AGTAAGG3550.075.532951
AAGGGAT15150.075.3819354
GAATAGG3500.075.267931
ATAACGG507.094059E-1175.267931
Produced by FastQC (version 0.11.5)