FastQCFastQC Report
Mon 27 Feb 2023
SRR3128460.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128460.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences761159
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATGCTGTG68320.8975785611153517No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64360.8455526374909841No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG37320.49030491658116104No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC33040.4340748779164406TruSeq Adapter, Index 15 (95% over 21bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA29820.3917709703228892No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28380.37285245264130096No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG26520.34841603396924953No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATG23110.30361593307048856No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCATGCTGTGTC15260.20048373598683059No Hit
CGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCT11750.15436984913795934No Hit
ACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG11120.1460929976522645No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCATGCTGTGTCGT9180.12060555022012484No Hit
AGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCT8800.11561316360970574No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGC8350.10970112683420942No Hit
AAAGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTT7760.10194978972855868No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT34700.085.508461
ATAGCGG1100.085.496141
TAGACGG1000.084.6411741
AGGGATG13250.082.280315
AGGGAAT15500.081.857015
AGGGCAT6250.080.450285
ACGGGTA1000.079.8863754
GGAGCTA30500.079.809349
TAGCGGG2950.079.647442
CGTAGGG2150.078.684252
GGTAAGG3500.077.923631
AATGCCC10150.077.779849
TCCGCTA5750.077.689971
TAAGGGA7950.077.4333343
TAACGGG2550.077.398572
GACCGAT551.8189894E-1276.895988
AGTAAGG4100.076.842261
GAGGGTA5200.076.813824
CTACGAC5700.076.6711355
CGCTACG5700.076.6711353
Produced by FastQC (version 0.11.5)