FastQCFastQC Report
Mon 27 Feb 2023
SRR3128459.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128459.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838928
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80740.9624187057768963No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG42440.5058836991970705No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATGCTGTG37140.4427078366677474No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC36720.43770144756165014TruSeq Adapter, Index 15 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33320.3971735357503862No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG30270.36081761486087005No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA17660.21050674193732957No Hit
CGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCT12850.15317166669845328No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATG11310.13481490664276316No Hit
AGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCT9830.11717334503080121No Hit
ACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG9130.10882936318730571No Hit
AAAGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTT8880.10584936967177161No Hit
AGAGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTT8520.10155817900940248No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA8510.10143897926878111No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG1450.087.622641
ACGGGTA1100.085.450374
CGTTTTT36750.084.893951
ATAGCGG1500.084.701881
AGGGATG16600.080.971955
AGGGAAT16050.080.525665
CGTAGGG2050.080.263912
GGAGCTA35950.079.48439
CGGGTAT1600.079.308635
TAGGGTC1550.078.8348544
GGGAATG20500.078.405936
CGCTACG6650.077.740563
TAGGGTA3100.077.31884
TAGCGGG3650.077.279532
GCTACGA6700.077.1604164
GGAATGC10650.076.784997
AGGGCAT7100.076.784995
GGTAAGG4300.076.6037751
CTACGAC6750.076.588855
TAAGAGG5000.076.23171
Produced by FastQC (version 0.11.5)