FastQCFastQC Report
Mon 27 Feb 2023
SRR3128458.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128458.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2205103
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182460.8274443416021836No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG35740.16207859678210043No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG35260.15990182771507727No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC32770.14860983817989454No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACCCGTAA28550.1294724101323158No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA22950.10407677101704547No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69800.082.73151
GTACGGG3200.079.345322
ACGGGTA2600.077.724154
CGTAGGG6050.070.723472
TAAGGGA20800.070.719943
AGTAGGG34450.070.426822
ATAAGGG28050.068.056682
TAGAGGG38400.067.468022
ATAGAGG14300.067.139151
AGTAAGG15250.066.351441
AGGGATG32050.066.131655
ATAGCGG3500.065.888521
ATAGGGC11850.065.834053
GAATAGG12550.065.251011
AGGGAAT28400.065.198675
AGAGGGC29450.064.629473
GAGGGAT28200.064.494514
CGAAGGG21900.064.410212
AAGGGAT36100.064.310314
GGATAGG14800.064.234951
Produced by FastQC (version 0.11.5)