FastQCFastQC Report
Mon 27 Feb 2023
SRR3128457.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128457.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2100015
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168710.8033752139865667No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG39720.18914150613209907No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG39340.18733199524765298No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC38410.18290345545150868No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACCCGTAA37070.17652254864846204No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA26100.12428482653695332No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59500.082.2700651
ACGGGTA2450.074.815114
TAAGGGA18150.073.79873
GTACGGG3000.073.628632
ATAGGGC9500.071.733973
CGTAGGG4750.070.2481462
TAGGGCG2950.070.1005554
AGTAGGG31900.069.68522
TAGGGCA9600.069.519714
CGTACAC18200.069.4659044
CCGTACA18300.069.084663
GAATAGG11450.068.994091
GTAGGGC9100.068.689553
TCCGTAC19250.067.6268542
ATAGCGG3550.067.553811
AGTACGG2450.067.175361
GGTAAGG12550.067.0683061
AGGGATG30900.066.926075
ATCCGTA19900.066.162671
ATAGGGA17000.066.073833
Produced by FastQC (version 0.11.5)