FastQCFastQC Report
Mon 27 Feb 2023
SRR3128456.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128456.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2333269
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208610.8940675078612882No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG46130.19770545102172102No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG43840.1878908947060969No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC42970.18416222047265018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71100.083.4530941
GTACGGG3250.076.656992
ACGGGTA2950.073.2859344
CGTAGGG6200.072.026312
GAATAGG12750.071.226911
AGTAGGG35000.069.1669162
TAGCGGG12600.068.271552
TAAGGGA21300.068.1810463
TGTAGGG16000.067.865842
AGGGATG32000.067.85425
ATAAGGG30550.067.08642
AGTAAGG13350.066.615841
TAGGGCA11100.066.475484
AAGGGAT36950.065.759864
ATAGGGC12450.065.3072743
AGAAGGG79700.065.231312
TAGAGGG40800.064.634142
ATAGGGA22250.064.4250263
AATAGGG32200.064.378672
TAGGGAT17450.064.101234
Produced by FastQC (version 0.11.5)