FastQCFastQC Report
Mon 27 Feb 2023
SRR3128455.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128455.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123651
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84510.7521018536894463No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC52790.46980779619294605No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCGACATC48370.43047173900081076No Hit
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG37490.3336445212970931RNA PCR Primer, Index 41 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC36970.3290167498627243RNA PCR Primer, Index 41 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG31980.28460794321368466Illumina PCR Primer Index 11 (95% over 24bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA22630.20139705299955235No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATG13690.12183498257021086No Hit
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT13430.11952109685302643RNA PCR Primer, Index 41 (95% over 24bp)
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCG12070.10741769464006172No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT43400.086.795531
ACGGGTA1050.085.041254
TAGGGCG1800.083.54934
CGCTACG9650.082.3047263
GCGGGAT6400.082.243844
CGTAGGG2200.081.251672
CGGGTAT1100.081.175745
CTACGAC9800.081.044965
GCTACGA9800.081.044964
GTACCGG353.815512E-880.719851
TCCGCTA10100.080.187051
AGGGATG29250.079.693195
ATAGCGG1900.079.303721
AGGGATT17400.079.1377645
TAACGGG3350.078.634772
TAGCGGG4300.077.671432
CGAGGGA7200.077.022023
AAGGGAT30600.076.945234
CGAAGGG10200.076.094832
TACGACC10600.074.928356
Produced by FastQC (version 0.11.5)