SRR3128454.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3128454.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1086502
Sequences flagged as poor quality	0
Sequence length	100
%GC	43

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7823	0.7200170823431525	No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCGACATC	6502	0.5984342412623262	No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC	5202	0.47878420840458646	No Hit
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	4370	0.40220818737563296	RNA PCR Primer, Index 41 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	4154	0.3823278742238854	RNA PCR Primer, Index 41 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	3681	0.33879366996103094	Illumina PCR Primer Index 11 (95% over 24bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA	2833	0.26074503314305914	No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATG	1953	0.17975116474705063	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	1478	0.13603288351056878	RNA PCR Primer, Index 41 (95% over 24bp)
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTCGACATCTC	1366	0.12572457298744044	No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCG	1202	0.11063026115000249	No Hit
AGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	1157	0.10648852924338842	RNA PCR Primer, Index 41 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	1151	0.10593629832250655	Illumina PCR Primer Index 11 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
ATAGCGG	155	87.97614	1
CGCTACG	900	86.68906	3
CGTTTTT	3970	86.463135	1
TCCGCTA	935	84.99115	1
GCTACGA	945	82.561005	4
TAACGGG	325	82.43093	2
GCGGGAT	600	82.25015	4
CTACGAC	950	82.122696	5
TACGACC	965	80.846176	6
AGGGATG	2590	80.38641	5
AAGGGAT	2675	80.295494	4
AGGGATT	1740	79.95049	5
AGGGCAT	985	79.68177	5
GACCGAT	95	79.150764	8
ACGACCA	975	78.5709	7
TAAGGGA	1235	78.39692	3
CGTAGGG	210	78.33348	2
TAGGGCA	500	78.02016	4
CGAAGGG	935	76.90924	2
GGAGCTA	2185	75.924515	9