FastQCFastQC Report
Mon 27 Feb 2023
SRR3128452.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128452.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008092
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480672.393665230477488No Hit
CGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT48560.24182158984747712No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTT45270.2254378783442193No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCT40850.20342693462251732No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG38390.19117649988147953No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC26720.13306163263436138No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC20700.10308292647946408No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT163950.088.15451
TACGGGT1700.080.171363
AGGGATG35600.075.644055
GTAGGGC9750.072.303083
AAGGGAT37000.070.368484
TAAGGGA17400.069.68523
GGTAAGG11150.069.644241
GAATAGG11700.069.5883261
GTACGGG5000.069.581542
TAGGGCA9550.069.388244
ATAGCGG4750.069.355441
ATAGGGC11050.069.325893
ACGGGAT7950.069.16544
AGTAGGG29750.068.902012
TAGCGGG11850.068.637292
ACGGGTA2200.068.359274
TAGAGGG35500.068.3366552
TAGGGAT15550.068.304324
ATAGGGA22000.068.1456453
GAGGGAT28950.067.85754
Produced by FastQC (version 0.11.5)