FastQCFastQC Report
Mon 27 Feb 2023
SRR3128449.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128449.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2529980
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234530.9270033755207552No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC34280.13549514225408898TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG31880.1260089012561364No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG30770.12162151479458336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79800.082.0387651
GTAGGGC10300.076.656433
TAGGGCA13850.073.296034
ATAGGGC15600.072.60543
AGTAGGG41750.071.642232
CGTAGGG6750.070.369752
ACGGGAT8550.070.3591164
ATAGGGA24250.069.963563
AGGGATG39450.069.454125
TAAGGGA22400.069.447483
ACGGGTA3600.069.191044
GGTAAGG15600.068.183421
CATAGGG12800.066.8697742
GAATAGG15000.066.831811
TAGAGGG48650.066.7013552
CGAAGGG25550.066.63252
GTACGGG7000.066.512852
AGGGAAT29700.066.303135
ATAGCGG7250.066.21511
GAGGGTA13700.066.208424
Produced by FastQC (version 0.11.5)