FastQCFastQC Report
Mon 27 Feb 2023
SRR3128448.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128448.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2397038
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216650.9038238025429718No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC39050.16290939067298893TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG36330.1515620528335387No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG36290.15139518021825268No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66550.082.828241
ACGGGAT7200.071.802884
GTAGGGC10800.071.801383
CGTAGGG6600.071.2079852
ACGGGTA3200.070.4973754
AGTAGGG38050.069.785692
ATAGGGC15850.068.7909553
ATAGGGA21700.068.6553
TAGGGCA14400.068.539114
AGGGATG35700.068.458265
TATAGGG18500.067.828482
TAGGGAT14600.067.600224
TAAGGGA23200.067.052133
GGTAAGG14050.066.95031
AGGGCAT22050.066.9286045
GTAGGGA18250.066.439973
AGGGCAC19600.066.422385
TCCGTAC9350.066.349082
GAATAGG15050.065.939381
AGGGAAT24950.065.74245
Produced by FastQC (version 0.11.5)