FastQCFastQC Report
Mon 27 Feb 2023
SRR3128447.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128447.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2659152
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262660.987758503462758No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC43790.16467655854197127TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG41470.15595197265895294No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG38050.1430907296762276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84300.080.868821
ATAGGGC16750.072.6721043
CGTAGGG7350.072.269582
TAGGGCA15200.072.043544
ATAGGGA24350.071.414353
AGTAGGG46900.071.0620652
GTAGGGC13850.070.921723
AGGGATG40150.070.4682855
TAGACGG3450.069.549081
ACGGGAT9250.067.576034
TAAGGGA25300.067.060913
GAATAGG17250.066.548921
TAGCGGG15500.066.4166262
ATAAGGG34800.066.188312
GTAGGGT12150.066.145843
TAGAGGG49950.065.781852
TAGGGAT17250.065.661484
ACGGGTA3150.065.6485064
AAGGGCA45000.065.2755054
AGGGCAC20900.064.988175
Produced by FastQC (version 0.11.5)