FastQCFastQC Report
Mon 27 Feb 2023
SRR3128446.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128446.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397679
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62770.4491016893006191No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTGTGAGA40720.29134014319453894No Hit
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG29290.2095617090905709Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC28010.20040366922590955Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG24900.17815249424224017Illumina Single End Adapter 1 (95% over 21bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA17070.12213104725763212No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT45950.085.618661
CGAAGGG10100.081.000332
CGTAGGG3000.078.362392
ACGGGAT7650.078.0187764
AGCGGGA11450.077.573463
GCGGGAT7450.076.959184
TAAGCGG3000.076.8639141
CGGGATT5500.076.047365
ATAGCGG3900.076.019251
CGAGGGA9050.075.8160553
AAGCGGG13600.075.711892
AGGGATT28000.075.360745
TAGGGCG3200.074.8991854
AAGGGAT35900.074.747824
CGAGGGT3500.073.8501743
TAGCGGG8300.073.6417542
ACGAGGG10850.072.367842
ATAGGGC10850.072.334193
AACGAGG4300.072.230921
AGGGCAC11750.071.993215
Produced by FastQC (version 0.11.5)