FastQCFastQC Report
Mon 27 Feb 2023
SRR3128444.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128444.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482347
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71710.4837598753868021No Hit
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG37570.2534494285076301Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC35210.23752872977784553Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG32110.21661594754804375Illumina Single End Adapter 1 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTGTGAGA27540.18578645890604562No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50950.085.334141
CGAAGGG12950.082.391422
CGGGTAT2250.077.283455
AACGAGG5200.076.914921
GGACCGA1350.076.5872047
ACGAGGG12950.076.221142
ACGGGTA1800.075.7168964
TAGGGCG2500.075.194714
AGGGCAC12400.074.2850955
AAGGGAT37550.073.467544
TAAGCGG3600.073.1948551
ACGGGAT7450.073.1760564
AGCGGGA11050.072.727913
AGGGATT28050.072.379945
GCGGGAT8800.072.09724
CGAGGGA11550.072.0209053
GCGAAGG6800.071.964681
AGCGGGT6400.071.9636843
TAGCGGG7850.071.8517762
ATAGCGG3300.071.2936861
Produced by FastQC (version 0.11.5)