FastQCFastQC Report
Mon 27 Feb 2023
SRR3128443.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128443.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2646408
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290141.0963540013482427No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG30400.11487268780928715No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102150.083.748091
CGTAGGG7250.075.883432
TAGGGCA13100.073.544784
ATAGGGC13950.071.758723
TAGGGTA9200.069.984534
AGGGATG39950.069.171925
ATAGCGG5950.068.805271
TATAGGG20900.068.6203162
AGGGAAT26950.068.533535
CCGTACA10350.068.1114653
AGTAGGG41950.067.141962
ATAGGGA22200.066.049713
CGAAGGG26350.066.0267942
TCCGTAC11150.065.788332
TGTAGGG21650.065.591592
TAGCGGG15000.065.517022
AGGGCAT22300.065.332035
AAGGGTA18650.065.266494
AGATAGG15000.064.9379351
AAGAGGG86400.064.818232
Produced by FastQC (version 0.11.5)