FastQCFastQC Report
Mon 27 Feb 2023
SRR3128442.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128442.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470024
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269591.091446884726626No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG34740.140646406674591No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC29800.12064660100468659No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG27640.11190174670367575No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97450.082.469641
CGTAGGG6850.072.729692
ACGGGTA3050.070.885024
TAGGGTA9250.070.62684
GTAGGGC12150.068.855743
TAGGGCA10750.068.641644
CCGTACA12750.068.1958543
AGGGATG35900.068.075025
AGATAGG12900.067.804291
AGTAGGG38350.067.037592
ATAGGGA21200.066.730913
AGTACGG4600.066.449151
ATAGGGC11950.066.0750963
TAGAGGG41950.065.990252
TATAGGG19400.065.6544342
AGGGTAC11700.065.475765
CGTACAC13150.065.049224
TCCGTAC13750.064.9452442
AGAGGGC37100.064.862333
GGTAAGG15550.064.7168351
Produced by FastQC (version 0.11.5)