FastQCFastQC Report
Mon 27 Feb 2023
SRR3128441.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128441.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2768875
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331721.1980316915714866No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG38770.14002076655681459No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC34730.12543000316012823No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG29930.10809444268881767No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT106600.084.1360551
CGTAGGG8350.079.936932
TAGGGTA10150.071.306594
ATAGGGC13800.071.1774143
TATAGGG20650.068.743632
AGTAGGG43650.068.596312
GGTAAGG17450.068.494351
AGGGATG42000.067.810725
TAGGGCA13050.067.7052464
GTAGGGT12450.067.1932453
GTAGGGC11850.066.62943
TAGAGGG48550.065.4490052
CGAAGGG26650.065.436832
TAGCGGG14550.064.934972
TAAGGGA21950.064.8758543
GTAGGGA21400.064.786313
AGGGCAT23650.064.783065
ACGGGTA3700.064.780374
ATAGCGG5700.064.392641
AGGGTAC14950.064.130415
Produced by FastQC (version 0.11.5)