FastQCFastQC Report
Mon 27 Feb 2023
SRR3128440.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128440.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441072
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141870.9844754460568244No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAGCTCCA42960.29811140595334584No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG33310.23114736807043645No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG31150.2161585264303241No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC29370.20380661063430558No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA21600.1498884164011236No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT16770.11637170106698347No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50850.082.481381
GTAGGGC4850.073.643133
AGGGAAT16100.073.558875
ATAGAGG8150.073.352561
TAGGGCA5650.073.197214
AGGGCGA7000.073.179395
CGTAGGG4950.072.207992
ACGGGTA1500.072.060454
AGGGTAC5900.070.892185
AGTAGGG18800.069.544672
ACTAGGG6100.069.388832
GTAGGGT4200.069.374963
AGTAAGG8600.068.966991
ATAGGGT4300.068.8545153
TAGAGGG24650.068.685092
TAGGGTA5150.068.44074
GAATAGG8000.067.0786061
TATAGGG10250.066.989372
AATAGGG18600.066.246852
AGACGGG9800.066.226212
Produced by FastQC (version 0.11.5)