FastQCFastQC Report
Mon 27 Feb 2023
SRR3128439.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128439.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407872
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129230.9179101509228111No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAGCTCCA59120.4199245385944177No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG37340.26522297481589235No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG35570.2526508091644695No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC32020.22743544867715246No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA25110.1783542822074734No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATG16730.11883182562051095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT46850.082.8904651
AATAGGG17600.073.421412
CGTAGGG4550.073.3245852
CGTACAC25350.073.03594
CCGTACA25500.072.787973
AGGGAAT14650.072.499535
TCCGTAC27050.072.265122
AGTAGGG19550.072.106962
ACGGGTA850.071.869154
GGAGCTA27750.071.637729
ATCCGTA27700.070.946441
GTAGGGC4650.070.737183
TATAGGG8450.070.623582
ATGTAGG3600.070.522121
AGACGGG10100.069.786682
GGTAAGG6500.069.4371641
AGTAAGG7900.069.034311
ATAGGGA11100.069.002893
TAGGGCA5050.068.858644
GAATAGG7450.068.786681
Produced by FastQC (version 0.11.5)