FastQCFastQC Report
Mon 27 Feb 2023
SRR3128437.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128437.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2329090
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198010.8501603630602509No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG23930.10274399014207265No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC23710.10179941522225418No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70900.080.098481
ATAGCGG5150.071.260791
ATAGGGC13500.071.0159453
TAGCGGG13150.068.285052
TAGGGCA14150.068.085854
GAATAGG15500.067.99541
AGATAGG15500.067.691851
AGTAAGG14950.067.664441
AGTAGGG37950.067.019862
GGATAGG14400.066.981461
AGGGATG31800.066.9469155
TAGACGG3100.066.78121
ATAGGGA22400.065.8781053
ATGTAGG7400.065.489061
CGTAGGG5350.065.0273362
CGAAGGG23450.064.5551762
GATAGGG37100.064.3736042
AATAGGG34300.064.146032
AAGGGCA36250.063.7847024
AGGGCAC18650.063.753085
Produced by FastQC (version 0.11.5)