FastQCFastQC Report
Mon 27 Feb 2023
SRR3128436.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128436.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2200497
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181170.8233140058813987No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG27230.12374477220373396No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG25290.11492858204305664No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC24850.11292903375919167No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT63350.079.7184451
GGTAAGG13300.070.356241
GAATAGG11550.070.024121
CCGTACA11500.069.8815463
GTAGGGA15650.068.767843
TAGCGGG12400.068.220532
AGTAGGG36300.067.969952
TAGGGAT13400.067.68884
CGTACAC11850.067.420954
ATAGCGG4750.067.315721
ATAGGGC11250.067.257023
ATAGGGA19250.067.137663
TCCGTAC12400.066.704512
TAGGGCA11700.066.67864
AGATAGG12950.066.448091
TAAGGGA19900.066.361713
AGTAAGG14850.066.179121
TAGAGGG38300.065.5249562
ACGGGTA2300.065.3862464
AGGGATG27550.064.993165
Produced by FastQC (version 0.11.5)