FastQCFastQC Report
Mon 27 Feb 2023
SRR3128435.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128435.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2480444
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223180.8997582690840833No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG31590.12735623138438118No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG28100.11328616973412824No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC27460.11070598650886695No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74100.080.4541
ATAGGGC13750.073.828413
AGATAGG14250.070.992571
CGTAGGG6750.068.9390262
ACGGGTA2050.068.776464
AGTAAGG15250.068.188581
AGTAGGG40950.067.951892
GGTAAGG14100.067.409561
TAGGGCA13950.067.042674
TAACGGG8150.066.901222
GAGGGAT29900.066.644934
ATAGGGA23700.066.2324143
AAGAGGG92500.066.110322
TAGCGGG15600.065.684912
TAGAGGG40150.065.442512
GAATAGG14550.065.324731
GATTAGG8100.065.0612261
AATAGGG34100.064.647562
AAGGGCA35550.064.51384
ATAGCGG5400.064.480321
Produced by FastQC (version 0.11.5)