FastQCFastQC Report
Mon 27 Feb 2023
SRR3128434.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128434.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2485087
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203620.8193676921572566No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC34030.1369368557318114No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG33430.13452245333865576No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG32860.13222877106515787No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72800.078.557721
CGTAGGG6950.073.0754852
ATAGGGC15850.072.345853
TAGGGCA13800.071.8549044
TAGAGGG44700.071.221962
ATAACGG2200.070.604951
TATAGGG21350.070.483072
ACGGGAT7450.070.0196154
AGTAGGG42950.069.525372
TAGTAGG10350.069.1269151
GGTAAGG14650.068.43621
AGGGATG40250.068.069995
ATAGAGG18400.067.7909851
GTAGGGC12350.067.73393
GAATAGG17250.067.125871
AGGGCAT22100.066.984045
ATAAGGG33000.066.975612
CGAAGGG25900.066.8160552
AGTACGG3600.066.682451
AATAGGG37300.066.314682
Produced by FastQC (version 0.11.5)