FastQCFastQC Report
Mon 27 Feb 2023
SRR3128433.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128433.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2303854
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186560.8097735359966387No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC39080.16962880460306948No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG37810.16411630250875273No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG36550.15864720594273768No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA23890.10369580711277711No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66900.079.0814361
TAGGGCA10250.069.693854
ATAAGGG31700.069.087792
TAAGGGT10650.068.84143
AGGGATG38500.068.602645
ATAACGG2200.068.403051
ATAGGGA19950.068.3171543
GGTAAGG13600.067.774341
ATAGCGG5700.067.6530151
ATAGGGC12600.067.5122763
ATAGAGG15850.067.3511051
AGTAGGG40750.067.238132
ACGGGAT7200.066.579784
TAGAGGG42500.065.796492
AGGGCAT20050.065.396585
GAGGGAT31550.065.3942954
AAGAGGG81450.065.3752752
AGAGGGC33300.065.3448643
AGGGAAT26400.065.3322145
TATAGGG18200.065.331682
Produced by FastQC (version 0.11.5)