FastQCFastQC Report
Mon 27 Feb 2023
SRR3128432.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128432.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2579678
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229120.888172864985475No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC43470.16850940311155113No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG41070.1592059163973178No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG40850.15835309678184642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77950.080.402481
ATAAGGG35950.072.042312
GTACGGG6150.071.079242
ATAACGG2550.070.117231
ATAGGGC15850.069.385873
AGGGATG41700.068.750875
TATAGGG20550.068.6192
AGTAGGG43450.068.585832
TAGAGGG48250.068.3871842
TAGGGCA15700.068.252674
CGTAGGG6000.067.372422
TAAGGGA25950.067.192533
AGATAGG13400.067.067161
GTAGGGC11400.066.787413
ATAGAGG18850.066.6470951
AGGGAAT31800.066.211795
TAGGGTA9800.066.1816254
AAGAGGG90000.066.014522
GGTAAGG16750.066.013751
GAATAGG15600.065.752651
Produced by FastQC (version 0.11.5)