FastQCFastQC Report
Mon 27 Feb 2023
SRR3128431.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128431.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335884
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178580.7645071416217586No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC29550.12650456957622896No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG27700.11858465574489145No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG25630.10972291432280028No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65700.080.251091
TAGGGCA12000.074.4107364
AGGGATG39800.074.272975
GAGGGAT31500.072.060924
CGTAGGG6500.070.8968662
AGGGCAT22100.070.8133545
AGTAGGG37450.070.315412
ATAGGGC13100.069.59753
TAAGGGA21250.068.338083
TAGAGGG42850.067.599612
AGAGGGC32650.067.507643
GTAGGGC9100.066.621063
AAGGGAT42000.066.4661254
AAGAGGG76650.066.25612
AAGGGCA34850.066.21284
GATAGGG34650.065.547812
GTAGGGA18050.065.091773
ATAGGGA21850.064.955923
AATAGGG31850.064.814092
ATAAGGG25850.064.759542
Produced by FastQC (version 0.11.5)