FastQCFastQC Report
Mon 27 Feb 2023
SRR3128430.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128430.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185146
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165930.7593542948617621No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC33830.15481803046569886No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG32350.14804502765490268No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG29070.13303458899313822No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCGCTAAAT24180.11065622159800764No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59550.079.35111
AGGGATG34650.073.91855
GTAGGGC10500.071.611153
ATAGGGC12700.071.417323
GTAGGGA19150.070.9216843
AGTAGGG35450.070.788882
TAGGGCA9800.070.4922264
TGTAGGG14800.070.173092
CGTAGGG7300.070.168742
AAGGGTA14900.069.703724
AGGGTAC10700.069.3958055
TAGAGGG39800.068.011052
ATAGGGA19650.067.6821063
ATAAGGG25450.067.582362
AAGGGAT40150.067.068574
TAAGGGA20200.066.769873
GAGGGAT27600.066.746274
AGTAAGG13600.066.724771
AAGAGGG70950.066.6986542
GGTAAGG13550.066.6239851
Produced by FastQC (version 0.11.5)