FastQCFastQC Report
Mon 27 Feb 2023
SRR3128429.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128429.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2441182
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205670.8425017061407138No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC36390.1490671322334836No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG35140.14394666190394653No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG34230.14021895950404353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68850.082.441681
AGGGATG40050.072.990825
CGTAGGG6350.072.541422
AGTAGGG39200.071.584992
GTAGGGC10000.070.4971543
TAGGGCA12350.068.8798144
GAGGGAT33650.068.4370654
AAGAGGG72350.067.955852
AGGGCAT20900.067.686275
TAGAGGG45350.067.681452
GGTAAGG13400.067.437261
AAGGGAT46350.067.429864
ATAGGGA25050.066.791723
TAAGGGA23750.066.489953
ACGGGTA2900.066.4455954
AGGGTAC10400.066.4300165
AAGGGCA33500.066.077944
AGGGATT26650.065.956075
GTAGGGA19450.065.483223
ATAGAGG16550.065.408371
Produced by FastQC (version 0.11.5)