FastQCFastQC Report
Mon 27 Feb 2023
SRR3128428.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128428.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2622037
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224960.8579589075211372No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC51750.19736563595403114No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT82700.081.36031
ATAGGGC16100.072.3925863
ATAGGGA28050.071.207273
AGGGATG44250.071.052785
ACGGGTA3500.069.8238144
ATAGAGG19250.069.41761
AGTAGGG43000.069.104672
AGGGATC25200.068.630255
TAGAGGG52000.068.265672
AAGAGGG89000.067.990362
AGGGTAC14900.067.498765
TAAGGGA24600.067.438493
GAGGGAT39950.067.171894
AAGGGAT52550.067.163834
CGTAGGG7800.066.306622
GAATAGG16050.066.254511
TAGGGCA13500.066.143644
TAGCGGG15500.065.824392
AGAGGGC37900.065.597113
CGAAGGG30200.065.0770952
Produced by FastQC (version 0.11.5)