FastQCFastQC Report
Mon 27 Feb 2023
SRR3128427.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128427.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2485368
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209870.8444222344538113No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC48990.19711366686945356No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC28500.11467114729086397No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG25750.10360638746455253No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75100.080.951561
ATAGGGA25850.072.900743
AGGGTAC14400.072.1265955
ATAGGGC12350.071.9188843
CGAAGGG25400.071.2322
CGTAGGG6950.070.999062
AGGGATG42400.070.8272865
TATAGGG17300.070.35612
GAATAGG13700.070.01261
TAGGGTA8400.069.373014
TAGGGCA12550.069.274934
TAGACGG3000.068.960121
GAGGGAT35450.068.801724
TAGAGGG44950.068.5839162
AGTAGGG44350.068.3461762
GTAGGGA20950.067.744033
AGGGAAT24650.067.3012855
ATAAGGG33500.067.195292
AGATAGG15450.066.6472551
AAGGGAT48500.066.5676354
Produced by FastQC (version 0.11.5)