FastQCFastQC Report
Mon 27 Feb 2023
SRR3128426.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128426.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2816465
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257210.9132369832396283No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC59110.2098730145767833No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC30290.10754616158908419No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG28180.1000545009435587No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93050.082.941451
ATAGGGC17150.075.360163
AGGGTAC16050.070.569215
CGTAGGG9000.070.501012
TAGGGCA14600.070.17414
TAGAGGG52550.070.1208952
AGTAGGG49550.069.908172
AGGGATG48500.069.1878365
ACGGGTA2950.068.504594
ATAAGGG37650.068.160342
GAGGGAT39850.067.57714
GTAGGGC13050.067.344843
GGTAAGG16500.067.308851
AAGAGGG98950.066.784192
AGTAAGG16800.066.3870241
TAGCGGG15300.066.353892
TAAGGGA24300.066.33783
TATAGGG22600.066.133692
AAGGGAT56300.065.612624
AGAGGGC41600.065.4121553
Produced by FastQC (version 0.11.5)